HILPADA Knockout cell line (HCT116)

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the HILPADA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, HILPADA gene knockout HILPADA Knockout cell line (HCT116) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Catalog#

YKO-KP270

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

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HILPADA Knockout cell line (HCT116)

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